流行性乙型脑炎病毒血清抗体半微量空斑抑制法的改进

本文报道一种检测流行性乙型脑炎(简称乙脑)中和抗体的简化空斑抑制试验方法,用BHK21传代细胞在24孔塑料板上进行试验,在细胞未形成单层时,将病毒直接接种在刚消化的细胞悬液内,孵育后加甲基纤维素复盖物,再培养,细胞层经染色后即可计算空斑数。 用该法检查47名儿童乙脑疫苗免疫前后的中和抗体,与常规的鸡胚细胞全量法或BHK21传代细胞微量中和试验法作了比较,结果表明本法与其它常规法的敏感性一致,且具有简便快速、节省人力和原材料、空斑形成率高的优点,便于大量血清学检测。     

Spatial relationship among individuals of the Japanese lacertidTakydromus tachydromoides (Sauria,Lacertidae)

The home range ofTakydromus tachydromoides was studied in a grassland area from April 1977 to November 1978. The mean size of home range did not differ markedly between sexes; 136.5 m² for males and 130.8 m² for females. Home ranges of adults overlapped greatly in each sex, and the lizard was considered to be non-territorial. Individuals showed return movement to a definite area (sleeping site) within the home range, and the home range did not shift within a year or between years. Characteristics of the home range of this grassland-inhabiting lizard were discussed in relation to resource abundance and predation pressure.     

Multivariate analyses of Canadian and Japanese populations ofGlycyphagus destructor (Schrank) (Acarina,Glycyphagidae)

Summary Two multivariate statistical procedures were used to determine the basic trends of morphologic and geographic variations between males of a common stored-grain mite,Glycyphagus destructor (Schrank) collected from Canada and Japan. All analyses were carried out on physical measurements of 25 morphological features. Three principal component analyses bases on the Canadian (50 specimens), Japanese (50 specimens) and the combined populations from the 2 countries (100 specimens) revealed that the first component, accounting for 40% of the variability in all 3 solutions represented a measure of the morphologic dimension of the body. The second component, which explained over 18% of the variability, is a measure of the leg dimension. Smaller clusters of variates characteristic of the population from each county were also evident. Discriminant analysis, performed with the Canadian and Japanese populations, identified the variates that differed between the 2 populations and provided an approximate appraisal of interrelations. The general conclusion based on these analyses is that the Canadian and the Japanese populations are morphologically distinct. The difference is most evident in the diameters of genu 2, lengths of the sensory seta W_I, lengths of the body, and the distances between the vertical external setae. Contribution No. 446, from Research Station, Canada Department of Agriculture, Winnipeg, Manitoba, Canada.     

Copulatory behavior unaccompanied by ovulation in the Japanese monkey (Macaca fuscata)

Copulatory behavior unrelated to conception is sometimes observed in some non-human primates including the Japanese monkey. In the present study, the authors examined whether a mature follicle or a newly formed fresh corpus luteum was observed in the ovaries of female Japanese monkeys which displayed the copulatory behavior unrelated to conception. Post-conception copulatory behaviors were observed in three out of four females usually kept in individual cages in an air-conditioned room, and in two out of three females without infants kept in an outdoor group cage. However, neither a mature Graafian follicle nor a fresh corpus luteum formed newly after conception was observed in any of these females by laparoscopic examinations conducted immediately after termination of the copulatory behavior. In females with infants born in the preceding birth season, copulatory behaviors were observed in three out of four females kept in the outdoor group cage, and in two out of four females in a free-ranging troop. Ovulation was confirmed in one case out of the three kept in the outdoor group cage, but neither a mature follicle nor a newly formed corpus luteum was observed in the remaining four females. These findings suggest that copulatory behavior in the Japanese monkey is not always controlled by the development of a follicle or ovulation in the ovary.     

日本落叶松人工林种群自然稀疏规律的研究

一、前言日本落叶松(Larix kaempferi)原产日本。种群地理分布范围为北纬35°20′—38°10′,东经136°45′—140°30′,海拔高度为1000—2800m。1900年引入我国,以其较强的适应性“定居”下来,而且生长速度在抚顺地区远比长白落叶松(L.olgensis var.changpaiensis)、兴安落叶松(L.gmelinii)为快;我国黑龙江、吉林、辽宁、山东、山西、陕西、北京、河南、河北、江西、湖北、四川以及新疆等地都有人工栽培,而以吉林、辽宁东部栽培面积较大,在年平均气温2.5—12.0℃、年降水量500—1400mm 的水热条件下能正常生长。     

Seasonal changes in the spermatogenic epithelium of adult Japanese macaques (Macaca fuscata fuscata)

A histological study was undertaken to clarify seasonal changes in the spermatogenic epithelium of Japanese macaques. Testicular tissue samples were excised by biopsies from five adult laboratory-maintained males in mating and non-mating seasons. The samples were fixed with Bouin's solution, embedded in paraffin, and stained with PAS and hematoxylin. Microscopic observations on cross-sections of seminiferous tubules revealed that the seminiferous epithelium in the mating season was thicker than in the non-mating season. PAS-stained granules were found in some of the dark A-type spermatogonia, which significantly increased in the non-mating season. Spermatids of the steps preceding the appearance of the acrosomic cap in stages I to III were observed significantly more often than those in the step coinciding with the formation of the acrosomic cap in stage IV. In stage I, the ratio of mature spermatids or spermatozoa to immature spermatids in the mating season was higher than that in the non-mating season. These findings suggest that spermiogenesis, as well as spermatocytogenesis, is inhibited in the non-mating season.